Invitrogen™ Human TEK (TIE2), GST Tag Recombinant Protein

Product Code. 11593477

Brand: Invitrogen™ PV3628

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Recombinant, Cytoplasmic Assay Entry: Biochemical phospho-specific activity (no antibody), Biochemical terbium phospho-specific activity, Biochemical europium phospho-specific activity, Biochemical binding/kinetic System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Z'-LYTE™, Omnia™ Bulk Substrate.

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.

Specifications

• Accession Number: Q02763
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 50% glycerol, 0.5mM EDTA, 0.02% Triton X-100, 150mM NaCl, 2mM DTT and no preservative; pH 7.5
• Gene ID (Entrez): 7010
• Molecular Weight (g/mol): 61.5 kDa
• Name: Human TEK (TIE2), GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: AA517024; Angiopoietin-1 receptor; CD202B; CD202b antigen; EC 2.7.10; EC 2.7.10.1; Endothelial tyrosine kinase; endothelial-specific receptor tyrosine kinase; endothelium-specific receptor tyrosine kinase 2; endothelium-specific receptor tyrosine kinase Tie-2; hTIE2; Hyk; mTIE2; p140 TEK; protein-tyrosine kinase Tie2; STK1; Tek; TEK receptor tyrosine kinase; TEK tyrosine kinase, endothelial; TEKT1; Tektin-1; Tie2; tie-2; tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; tyrosine kinases that contain immunoglobulin-like loops and epidermal growth factor-similar domains 2; tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor Tie-2; VMCM; VMCM1
• Common Name: TIE2 (TEK)
• Gene Symbol: TEK
• KinaseFamily: TIE Kinase Family
• Protein Length: 817-1101
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 817-1101
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Assay Entry: Biochemical phospho-specific activity (no antibody), Biochemical terbium phospho-specific activity, Biochemical europium phospho-specific activity, Biochemical binding/kinetic
• Kinase Group: Tyrosine Kinases
• Shipping Condition: Dry Ice
• System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Z'-LYTE™, Omnia™ Bulk Substrate
• Target Entry: TEK
• Target Gene: TEK tyrosine kinase, endothelial
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Cytoplasmic
• Form: Liquid
• Protein Subtype: Receptor Tyrosine Kinases
• Research Category: Signal Transduction