TE buffer, pH 8.0, RNAse free, Thermo Scientific Chemicals

Product Code	Brand	Quantity
15445059	Thermo Scientific Alfa Aesar J62745.=E	125 mL
15455059	Thermo Scientific Alfa Aesar J62745.AK	250 mL

Description

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer. TE buffer is often used to store DNA and RNA while protecting it from degradation. Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer. TE buffer is often used to store DNA and RNA while protecting it from degradation. Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.

Solubility
Not miscible or difficult to mix in water.

Notes
200mM Tris-HCl with 20mM EDTA

Chemical Identifiers

• CAS: 1185-53-1
• Molecular Weight (g/mol): 157.594
• InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N
• IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride

• Molecular Formula: C4H12ClNO3
• MDL Number: MFCD00236359
• PubChem CID: 93573
• SMILES: C(C(CO)(CO)N)O.Cl

Specifications

• CAS: 1185-53-1
• Physical Form: Liquid
• MDL Number: MFCD00236359
• InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N
• IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
• PubChem CID: 93573

• pH: 8.0
• Molecular Formula: C4H12ClNO3
• Solubility Information: Not miscible or difficult to mix in water.
• SMILES: C(C(CO)(CO)N)O.Cl
• Molecular Weight (g/mol): 157.594
• Chemical Name or Material: TE buffer, RNAse free

Safety and Handling

missing translation for 'tsca' : Yes

Recommended Storage : Ambient temperatures

RUO – Research Use Only