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TCR DO11.10 Monoclonal Antibody (KJ1-26 (KJ126, KJI-26, KJI26)), Biotin, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 13-5808-82
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: The KJ1-26 anti-idiotypic monoclonal antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on any other T cell hybridomas tested.The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-A^{d^} major histocompatibility molecules.The use of DO11.10 and KJ1-26 have proven to be instrumental in describing many of the intricate details of T cell immunity. Applications Reported: This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been reported for use in flow cytometric analysis. Applications Tested: This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been tested by flow cytometric analysis of the DO11.10 T cell hybridoma. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.
TCR DO11 (DO11.10) is a receptor found on lymphocytes of DO11.10 transgenic mice and T-cell receptors of BALB/c-derived DO11.10 and DO11.10.24 T cell hybridomas. The DO11.10 TCR recognizes a C-terminal ovalbumin peptide (OVA 323-339) in the context of I-Ad major histocompatibility molecules. The use of antibodies against DO11.10 have proven to be instrumental in describing many of the intricate details of T cell immunity, including T cell activation, development, and function.Specifications
TCR DO11.10 | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P01849 | |
Tcra | |
Affinity chromatography | |
RUO | |
21473 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
KJ1-26 (KJ126, KJI-26, KJI26), KJI-26, KJI26) | |
Biotin | |
Tcra | |
D011.10; T cell receptor alpha chain; Tcra; Tcralpha | |
Mouse | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
For Research Use Only.