TCL1 Mouse anti-Human, Clone: eBio1-21 (1-21), eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-6699-82

100UG:ANTI-HUMAN TCL1 PURIFIED 100 UG

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only.

Product Code. 15247937

Quantity Price
1 £ 188.0 / 100µg
Estimated Shipment date
from Supplier 08-12-2016
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Description and Specification

Specification

Antigen TCL1
Applications Flow Cytometry (Intracellular Staining)
Applications Immunohistochemistry (Formalin/Paraffin)
Applications Immunoprecipitation
Applications Western Blotting
Clone eBio1-21 (1-21)
Concentration 0.5mg/mL
Conjugate Unlabeled
Format Purified
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias T Cell Leukemia/Lymphoma 1, TCL-1
Host Species Mouse
Isotype IgG2b
Quantity 100μg
Regulatory Status RUO
Species Reactivity Human
Storage Requirements Store at 2-8°C.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The eBio1-21 antibody reacts with human T cell leukemia/lymphoma 1 (TCL1), a 14kDa proto-oncogene product with a suggested role in intracellular regulation of T cell signalling. TCL1 was identified as the oncogene located at the 14q32.1 chromosome breakpoint region in T-cell prolymphocytic leukemia (T-PLL). In T-PLL, TCL1 is overexpressed as a result of an inversion or a reciprocal translocation, by juxtaposition to the T-cell receptor promoter/enhancer elements. TCL1 binds to the pleckstrin homology domain of Akt (protein kinase B) family proteins, which facilitates Akt dimerization and activity. By increasing Akt activity TCL1 may enhance the serine/threonine phosphorylation of major Akt signaling substrates, such as Ikk complex, mTOR, BAD, p70S6 kinase, FOXO transcription factors, and GSK3β. These substrates regulate cellular differentiation, growth, survival, and metabolism.

Besides its tumorigenic role in T-PLL, TCL1 is normally expressed in the CD3-CD4-CD8- subset of thymic precursors in the T cell lineage, the plasmacytoid subset of dendritic cells, stimulated (not resting) mature T cells, and B cells up to the germinal center stage of maturation. TCL1 is inappropriately expressed by chromosome rearrangements that lead to pre-malignant clonal T cell expansions and mature T cell tumors. TCL1 shows a regulated expression pattern in chronic lymphocytic leukemia (CLL).