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Thermo Scientific Chemicals TBE running buffer (10X)
Product Code. 15475089
Brand: Thermo Scientific Chemicals J62788.K7
Description
TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. TBE Buffer is used for polyacrylamide and agarose gel electrophoresis. It is ideally suited for DNA and RNA separation on longer run, higher voltage or amperage gels. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel.5 TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE).
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Specifications
Specifications
| Chemical Name or Material | TBE running buffer (10X) |
| Physical Form | Liquid |
| Quantity | 4 L |
| Packaging | Plastic bottle |
| Solubility Information | It is soluble in water. |
Frequently Asked Questions (FAQs)
Concentrations of 5X Tris Borate EDTA (TBE) buffer or greater may precipitate due to the nature of the product. This precipitation is a result of nucleation of salt crystals by dust particles or other insoluble materials. In some cases, we recommend heating the buffer to ~ 50°C to re-dissolve the TBE into solution. Alternately, to avoid precipitation of high concentration TBE buffers, after the solution is prepared, you can filter it through a 0.2 µm cellulose acetate or cellulose nitrate filter. These treatments have no deleterious effect on the buffering properties of TBE.
Safety and Handling
- TBE running buffer (10X)
- Danger
- Serious eye damage/eye irritation Category 2
- Reproductive toxicity Category 1B
- Skin corrosion/irritation Category 2
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H360FD-May damage fertility. May damage the unborn child.
- P201-Obtain special instructions before use.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- MIXTURE LIST-Contains : N,N-1,2-ethanediylbis[N-(carboxymethyl)-, disodium salt, dihydrate
RUO – Research Use Only
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