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TaqMan™ SCID/SMA Plus Assay
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No. of Reactions:
1000 Reactions
4000 Reactions
8000 Reactions
Unit Size:
1000 reactions
4000 reactions
8000 reactions
Description
The Applied Biosystems TaqMan SCID/SMA Plus Assay is a multiplex real-time PCR assay that detects the SMN1 gene, TREC, and KREC, as well as the RNase P gene as an internal genomic control. For an assay that detects only SMN1 and TREC, please see the TaqMAN SCID/SMA Assay (Cat. No. A48566).
Features of the TaqMan SCID/SMA Plus Assay include:
• Optimized for SCID and SMA
• Direct extraction from DBS samples for reduced hands-on time and cost
• Compatible with 96- or 384-reaction protocols
• High-quality results for 96 samples within 55 min using the fast cycling protocol
• Minimal liquid-dispensing steps for easy automation
Complete solution from DBS to result
The TaqMan SCID/SMA Plus Assay detects SMN1, TREC, and KREC, as well as the RNase P gene as an internal genomic control. The assay solution contains a mixture of primers and probes for each target plus an SMN2 blocker. From DBS to result, less than 40 min of hands-on time and only four reagent-dispensing steps are required for 96-well plates. One additional pipetting transfer step from 96- to 384-well plates is needed. A real-time PCR instrument with at least five filters is required to detect the reporter dyes plus the passive reference dye.
Features of the TaqMan SCID/SMA Plus Assay include:
• Optimized for SCID and SMA
• Direct extraction from DBS samples for reduced hands-on time and cost
• Compatible with 96- or 384-reaction protocols
• High-quality results for 96 samples within 55 min using the fast cycling protocol
• Minimal liquid-dispensing steps for easy automation
Complete solution from DBS to result
The TaqMan SCID/SMA Plus Assay detects SMN1, TREC, and KREC, as well as the RNase P gene as an internal genomic control. The assay solution contains a mixture of primers and probes for each target plus an SMN2 blocker. From DBS to result, less than 40 min of hands-on time and only four reagent-dispensing steps are required for 96-well plates. One additional pipetting transfer step from 96- to 384-well plates is needed. A real-time PCR instrument with at least five filters is required to detect the reporter dyes plus the passive reference dye.
Specifications
Specifications
| Content And Storage | • DNA Extract All Reagents Kit • TaqPath ProAmp Multiplex Master Mix • TaqMan SCID/SMA Plus Assay Upon receipt, store at -20°C. After opening, store at 4°C. |
| Detection Method | Primer-probe |
| For Use With (Equipment) | QuantStudio™ 12 k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, QuantStudio™ Dx System (in RUO Mode) |
| Product Line | TaqMan |
| Quantity | 1,000 reactions |
| Shipping Condition | Dry Ice |
| No. of Reactions | 1000 Reactions |
| Sample Type | DNA (Genomic), cDNA |
For Research Use Only. Not for use in diagnostic procedures.
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