TaqMan™, RNA-to-CT™ TaqMan™ RNA-to-C_T™ 1-Step Kit

Product Code. 10158014

Brand: TaqMan™, RNA-to-CT™ 4392653

Description

Delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems™ comprehensive library of TaqMan™ Gene Expression assays.

The TaqMan™ RNA-to-CT™ One-Step Kit is recommended for a variety of applications including general gene expression studies, biomarker discovery, and microarray validation.

• Accurate across a wide dynamic range for dependable performance
• High sensitivity, enabling low copy number detection and precise quantification
• Validated with TaqMan™ Gene Expression Assays for easy experimental setup
• Consistent performance with a wide variety of targets, including AT-rich, GC-rich and long sequences
• Fewer pipetting steps means less hands-on-time and less chance for errors

Efficiency across a Broad Range of Input Concentrations

• Maximum flexibility in RNA template input quantity requires optimal real-time PCR efficiency across a broad range of template quantities
• The result is comparable to that of the TaqMan™ RNA-to-CT™ 2-Step Kit, and is a significant improvement in dynamic range and sensitivity over the TaqMan™ One-Step RT-PCR Master Mix Reagents.

High Sensitivity at Low Target Concentration

• The sensitivity of TaqMan™ RNA-to-CT™ One-Step Kit was validated using a synthetic RNA template for which copy number is precisely known
• Significant sampling error occurs when measuring low quantities of target, so statistical analysis is required for proper evaluation using multiple replicates
• Statistical analysis indicates high confidence of sample quantification based on a T-test, consistent with as few as 10 copies of target.

Order Info

Shipping Conditions: Wet Ice

Specifications

• Content And Storage: Sufficient for 40 reactions at 50 μL reaction volume. Includes:
  • 1 x 1 mL tube of 2X master mix containing AmpliTaq Gold™ DNA Polymerase UP, dNTPs (including dUTP), Passive Reference 1, and optimized buffer components. Store at -15 to -25°C.
  • 1 x 50 μL tube of 40X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15° to -30°C.
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• Detection Method: Primer-probe
• PCR Method: 1-step RT-qPCR
• Polymerase: AmpliTaq Gold DNA Polymerase
• Reaction Speed: Standard
• For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
• Passive Reference Dye: ROX (Pre-mixed)
• Product Line: RNA-to-C_T™, TaqMan™
• Product Type: One-Step RT-PCR Kit
• Purity or Quality Grade: UP (Ultra Pure)
• Quantity: 40 reactions
• Reverse Transcriptase: ArrayScript™ UP
• Shipping Condition: Wet Ice
• Sufficient For: 40 Reactions
• For Use With (Application): Gene Expression
• No. of Reactions: 40 Reactions
• Sample Type: RNA

For Research Use Only. Not for use in diagnostic procedures.