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Applied Biosystems™ TaqMan™ Protein Assays Core Reagents Base Kit
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TaqMan™ Protein Assays Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan™ protein expression experiments. The Universal PCR Assay contains optimized concentrations of TaqMan™ probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems real-time PCR system.
Specifications
Specifications
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, AB 7900 HT 384-Well System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
| Product Line | TaqMan |
| For Use With (Application) | Proximity Ligation Assay |
| Content And Storage | The TaqMan™ Protein Assays Core Reagents Base Kit contains: DNA Ligase, 500X (1 × 10 μL) Ligase Dilution Buffer, 1X (2 × 1.5 mL) Ligation Reaction Buffer, 20X (1 × 0.7 mL) Phosphate Buffered Saline (PBS), 1X, pH 7.4 (1 × 1 mL) Protease, 100X (1 × 10 μL) Universal PCR Assay, 20X (1 × 120 μL) Store all components at -15 to -25°C. |
| Detection Method | Primer-Probe Detection |
| Label or Dye | FAM |
| Product Type | Reagents for Protein Expression |
| Quantity | 100 Reactions |
| Sample Type | Cells |
| No. of Reactions | 100 reactions |
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For Research Use Only. Not for use in diagnostic procedures.
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