Applied Biosystems™ TaqMan™ Protein Assays Core Reagents Base Kit

Product Code. 11521306

Brand: Applied Biosystems™ 4405460

Description

TaqMan™ Protein Assays Core Reagents Base Kit

TaqMan™ Protein Assays Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan™ protein expression experiments. The Universal PCR Assay contains optimized concentrations of TaqMan™ probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems real-time PCR system.

Specifications

• For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, AB 7900 HT 384-Well System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
• Product Line: TaqMan™
• For Use With (Application): Proximity Ligation Assay
• Content And Storage: The TaqMan™ Protein Assays Core Reagents Base Kit contains:
  
  DNA Ligase, 500X (1 × 10 μL)
  Ligase Dilution Buffer, 1X (2 × 1.5 mL)
  Ligation Reaction Buffer, 20X (1 × 0.7 mL)
  Phosphate Buffered Saline (PBS), 1X, pH 7.4 (1 × 1 mL)
  Protease, 100X (1 × 10 μL)
  Universal PCR Assay, 20X (1 × 120 μL)
  Store all components at -15 to -25°C.
• Detection Method: Primer-Probe Detection
• Label or Dye: FAM
• Product Type: Reagents for Protein Expression
• Quantity: 100 Reactions
• Sample Type: Cells
• No. of Reactions: 100 reactions
• Primer Probe Compatibility: TaqMan™
• PCR Method: qPCR Protein Quantification
• Reaction Speed: Fast
• Shipping Condition: Wet Ice
• Target Specificity: Not Target-Specific

For Research Use Only. Not for use in diagnostic procedures.