missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. Real Time PCR Products
  6. Real Time PCR Assays and Arrays
  7. Applied Biosystems™ TaqMan™ Pri-miRNA Assay

Applied Biosystems™ TaqMan™ Pri-miRNA Assay
GREENER_CHOICE

Product Code. 10321815 Shop All Applied Biosystems Products
Click to view available options
Quantity:
L (2900 reactions), made to order
M (750 reactions), made to order
S (360 reactions), made to order
Unit Size:
Each
This item is not returnable. View return policy

Product Code. 10321815

Brand: Applied Biosystems™ 4427012

Please to purchase this item. Need a web account? Register with us today!

253.50 GBP valid until 2025-12-31
BEST PRICE on Promo! Use promo code "24076" to get your promotional price.
This item is not returnable. View return policy

Description

TaqMan Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway.

TaqMan Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are designed using our state-of-the-art bioinformatics pipeline, delivering gold-standard assay performance and data quality for pri-miRNA studies.

• Highly specific—quantitiate miRNA transcription from a single genomic locus
• Fast, simple, and scalable—two-step RT-qPCR provides high-quality results
• A new perspective—measure miRNA expression at the gene level

TaqMan Pri-miRNA Assays apply the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan genomic assays are renowned to the detection of pri-miRNA transcripts. As a result of their high sensitivity, TaqMan Pri-miRNA Assays require as little as 1 ng of sample input to quantify expression from moderate- to high-expression miRNA loci.

Design and selection
Since all pri-miRNA transcripts have not been mapped, these assays are designed within close proximity to each stem-loop sequence represented in the Sanger miRBase sequence repository. This ensures accurate measurement of the transcript of interest. Each publicly available stem-loop can be matched to a TaqMan Pri-miRNA Assay and TaqMan MicroRNA Assay, enabling the RNA sequences produced at either end of the miRNA maturation pathway to be quantified independently.

Pre-formulated assay (20X; 60X mix)
• 2 unlabeled PCR primers (900 nM each final 1X reaction concentration)
• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X reaction concentration)

TRUSTED_SUSTAINABILITY

Specifications

Concentration 20X
Content And Storage 1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Reaction Speed Fast
Green Features Sustainable packaging
Product Line TaqMan™
Product Type Pri-miRNA Assay
Quantity S (360 reactions), made to order
Shipping Condition Room Temperature
Sufficient For 360 Reactions
Fidelity (vs. Taq) 60 X
No. of Reactions 360 reactions
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.