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Applied Biosystems™ TaqMan™ MicroRNA Assay

Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with an miRNA-specific primer, followed by real-time PCR with TaqMan probes.

£201.00 - £1760.00

Specifications

Content And Storage • 1 tube containing RT primer at concentration of 5X (XS and S sizes), 20X (M size), or 60X (L size)
• 1 tube containing a 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.

Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Reaction Speed Fast
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Products 5
Product Code Brand Description Price Quantity & Availability  
Product Code Brand Description Price Quantity & Availability  
10524815
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Applied Biosystems™
4427975
S (50 RT/150 PCR reactions), Inventoried
£201.00
150 reactions
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Estimated Shipment: 15-04-2024
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15187092
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Applied Biosystems™
4440887
M (750 RT/750 PCR reactions), Made to Order
£800.00
750 reactions
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Estimated Shipment: 02-05-2024
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15376731
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Applied Biosystems™
4440886
S (50 RT/150 PCR reactions), Made to Order
£339.00
Each
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Estimated Shipment: 07-05-2024
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15383712
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Applied Biosystems™
4440888
L (2900 RT/2900 PCR reactions), Made to Order
£1760.00
2900 reactions
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Estimated Shipment: 23-04-2024
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15312198
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Applied Biosystems™
4440885
XS (25 RT/75 PCR reactions), Made to Order
£229.00
Each
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Estimated Shipment: 16-04-2024
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Description

Description

Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with an miRNA-specific primer, followed by real-time PCR with TaqMan probes. TaqMan MicroRNA Assays are:

Highly specific—quantitate only mature miRNAs, not precursors
Sensitive—conserve limited samples, requires only 1–10 nanograms of total RNA or equivalent
Fast, simple and scalable—two-step quantitative RT-PCR assay provides high-quality results in less than three hours

Our TaqMan MicroRNA Assay catalogue is kept up to date with the Sanger database to provide comprehensive coverage with the most current annotation.

Availability and sizes
Made-to-Order assays are available in four different sizes and usually ship within two weeks. Inventoried assays are prepackaged in Small size and are ready to ship. Inventoried assays may be purchased in larger sizes by ordering them as Made to Order.

Species
TaqMan MicroRNA Assays are available for a range of species including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis. We will continue to increase the number of miRNA assays for these species in alignment with the Sanger miRBase Registry.

Assay Components
TaqMan MicroRNA Assays are delivered in two tubes: one containing the RT primer, the other containing the specific pre-formulated TaqMan Assay (TaqMan probe and PCR primer set).

Pre-formulated assay
• Forward primer: 1500 nM
• Reverse primer: 700 nM
• FAM-MGB probe: 200 nM
• RT sequences: 50 nM

TaqMan miRNA Assay selection guide

TaqMan MicroRNA Assays
Description: TaqMan MicroRNA Assays employ a novel target-specific stem–loop primer during cDNA synthesis to produce a template for real-time PCR
RT chemistry: miRNA-specific RT
Throughput: Best for 1–10 targets
Coverage: 205 species available, coverage for miRBase v.21

TaqMan Advanced miRNA Assays
Description: TaqMan Advanced miRNA Assays employ a universal RT step for a streamlined workflow, and a universal miR-Amp step to enable highly sensitive detection by real-time PCR
RT chemistry: Universal RT
Throughput: Best for >10 targets
Coverage: All human, mouse, and rat miRNAs; coverage for miRBase v.24

Specifications

Specifications

• 1 tube containing RT primer at concentration of 5X (XS and S sizes), 20X (M size), or 60X (L size)
• 1 tube containing a 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.

High
Fast
Sustainable packaging
Room Temperature
Tube
qPCR
7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
TaqMan™
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.