Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents

TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.

Code produit	Marque	No. of Reactions
10085164	Applied Biosystems™ 4308321	1000 Reactions
10753447	Applied Biosystems™ 4308323	200 Reactions

Description

• Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe
• The IPC can be spiked into samples to distinguish true target negatives from PCR inhibition
• This kit allows users to amplify a low-copy target DNA in the same tube with the IPC
• Although the target and IPC DNAs may differ in initial copy number, the concentration of the IPC primers in the PCR reaction is limiting so that the amplification efficiency of the target reaction is not compromised
• Pre-designed primers and TaqMan™ probe eliminate assay design
• Rapid assay development guidelines can minimize optimization time Separate components provide flexibility in assay set-up
• Reagents may be stored at 2°C to 4°C if they are to be consumed within one month
• Otherwise, store at -20°C to -25°C

Note: This is not a quantitative kit and should not be used for normalization Instead, this kit is designed to distinguish types of negative results: a negative call for the target sequence and a positive call for the IPC indicate that no target sequence is present, and a negative call for the target sequence and negative call for the IPC suggest PCR inhibition.

Informations sur la commande

Note: This is not a quantitative kit and should not be used for normalization. Instead, this kit is designed to distinguish types of negative results: a negative call for the target sequence and a positive call for the IPC indicate that no target sequence

Spécification

• Format: Tube(s)
• PCR Method: qPCR
• Green Features: Sustainable packaging
• Product Line: TaqMan™

• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Internal Probe Modification: TAMRA™ Quencher (3'), VIC™ (5')
• Product Type: Exogenous Internal Positive Control Reagent

For Research Use Only. Not for use in diagnostic procedures.