Applied Biosystems™ TaqMan™ Copy Number Assay

Product Code. 10752697

Brand: Applied Biosystems™ 4400293

Description

Applied Biosystems TaqMan Copy Number Assays use gold-standard TaqMan MGB probe chemistry to evaluate the copy number of genomic DNA targets using Applied Biosystems real-time PCR instruments and software.

Applied Biosystems TaqMan Copy Number Assays use gold-standard TaqMan MGB probe chemistry to evaluate the copy number of genomic DNA targets using Applied Biosystems real-time PCR instruments and software. TaqMan Copy Number Assays are run together with a TaqMan Copy Number Reference Assay in a duplex qPCR reaction; the copy number assay detects the target sequence, and the reference assay detects a sequence that is known to be present in two copies in the diploid genome. The results are then analyzed by the relative quantitation method using Applied Biosystems CopyCaller Software.

The predesigned TaqMan Copy Number Assay collection consists of human and mouse assay libraries for copy number variation (CNV) analysis. The human assay collection includes over 1.6 million assays targeting gene exons and introns, extragenic regions, and CNV sequences from the Database of Genomic Variants (DGV). The mouse collection includes over 180,000 assays targeting gene exons. Assays to common vector marker and reporter genes are also available for transgenic studies.

Benefits:

• Specific—gold-standard TaqMan chemistry with minor groove binder (MGB) probes increase assay specificity by enabling shorter probes
• Reproducible—robust assay designs developed with our bioinformatics pipeline enable accurate, reproducible, and reliable results
• Easy to interpret—CopyCaller Software provides the calculated copy number and predicted copy number, along with confidence value and z-score quality metrics
• Fast and simple—setup to primary analysis in 3–4 hours

Approximate ship time

4–6 days in North America and 6–10 days in Europe

All assay designs are the product of our bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

All of our predesigned TaqMan Assays are covered by the TaqMan Assays qPCR Guarantee.*

TaqMan Copy Number Reference Assays are sold separately.

Recommended master mix (sold separately): TaqMan Genotyping Master Mix (Cat. No. 4371355)

*Terms and conditions apply. For complete details, go to www.thermofisher.com/taqmanguarantee.

Specifications

• 3'Primer Modification: None
• 5'Primer Modification: None
• Concentration: 60X
• Content And Storage: 1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).
  
  Store at -15 to -25°C.
  
  
• Detection Method: Primer-probe
• Form: Frozen
• PCR Method: qPCR
• Reaction Speed: Fast
• For Use With (Equipment): 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
• Green Features: Sustainable packaging
• Internal Probe Modification: FAM (5'), MGB (Minor Groove Binder) (3')
• Label or Dye: FAM
• Product Line: TaqMan™
• Product Type: Copy Number Assay
• Purification Method: Solid Phase Extraction (SPE)
• Purity or Quality Grade: RNase-Free, DNase-Free
• Quantity: L (2900 reactions), made to order
• Shipping Condition: Room Temperature
• Species: Human
• No. of Reactions: 2900 reactions

For Research Use Only. Not for use in diagnostic procedures.