missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit

The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

£672.00 - £17570.00

Specifications

Content And Storage • Express Lysis Solution (room temperature or 4°C)
• Express ezDNase (–20°C)
• SuperScript IV VILO Master Mix (–20°C)
• SuperScript IV VILO No RT Control (–20°C)
• TaqMan Fast Advanced Master Mix (4°C)

Detection Method Primer-probe
Form Liquid
Format Kit
GC-Rich PCR Performance High
View More Specs

Products 4
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
17827058
View Documents Promotion Details
Invitrogen™
A57985
40 Reactions
£672.00
40 reactions
View alternative products
Save Up to 
»
Estimated Shipment: 14-05-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
17856758
View Documents Promotion Details
Invitrogen™
A57986
100 Reactions
£1565.00
100 reactions
View alternative products
Save Up to 
»
Estimated Shipment: 14-05-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
17857048
View Documents Promotion Details
Invitrogen™
A57987
400 Reactions
£5643.00
400 reactions
View alternative products
Save Up to 
»
Estimated Shipment: 14-05-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
17857038
View Documents Promotion Details
Invitrogen™
A57988
2500 Reactions
£17570.00
2500 reactions
View alternative products
Save Up to 
»
Estimated Shipment: 14-05-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
Description

Description

The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification. It enables RT-qPCR of the highest sensitivity and quickest workflow using just five components: Express Lysis Buffer, Express ezDNase, Express RT Master Mix, RT controls, and our best-in-class TaqMan Fast Advanced Master Mix. The TaqMan Cells-to-CT Express Kit provides the sensitivity you need in the fastest workflow available: RT-qPCR results for gene expression analysis in approximately 70 minutes.

Features of the TaqMan Cells-to-CT Express Kit include:
Extraordinary ease and speed—96 samples ready for RT-qPCR in as little as 5 minutes
No tedious RNA purification—no columns, heating, or centrifugation
Verified workflows—complete set of reagents optimized to work efficiently right out of the box
Environmentally friendly—the kit is REACH Compliant, has fewer components, and requires fewer pipetting steps reducing plastics costs
Right sized kits for automation—available in 2500 reaction size that includes both lysis and RT components

Optimized chemistry with no need for stop solution
Gene expression analysis from direct cell lysis is made simple with our TaqMan Cells-to-CT Express Kit. With only one step for cell lysis and no need for the addition of a Stop Solution, our Express technology enables a rapid workflow with sensitive, accurate, and reproducible results. 10–100,000 cells are lysed using the Express Lysis Solution. The optional inclusion of ezDNase to the Express Lysis Solution enables removal of genomic DNA during cell lysis. Following a 5-minute incubation at room temperature, the lysate is used directly for reverse transcription using SuperScript IV VILO Master Mix. Once the RT reaction is complete, qPCR analysis is carried out using TaqMan Fast Advanced Master Mix and a TaqMan Gene Expression Assay.

Streamlined workflow allowing for rapid, accurate and sensitive results
RNA prepared using the TaqMan Cells-to-CT Express technology substantially expedites and streamlines gene expression analysis. Cell lysates exhibit sensitivity and specificity better than or equivalent to RNA extracted using traditional spin column isolation methods. The lysis procedure simultaneously prepares cell lysates for RT-qPCR and removes genomic DNA in only five minutes. The lysis step is simple to automate with robotic platforms for high-throughput processing of 96- or 384-well plates because it takes place entirely at room temperature. The procedure is also economical as there are only a few pipetting steps and, for cells grown in 96- or 384-well plates, no sample transfers. The high efficiency SuperScript IV VILO RT Master Mix transcribes even the most difficult (degraded and low abundant) RNA while reducing the typical reaction time by 10 minutes. Additionally, the TaqMan Cells-to-CT Express Kit is compatible with over 700,000 TaqMan Gene Expression Assays.

Maximize sensitivity of detection
Unlike some competitor kits that limit the amount of lysate in the RT reaction to 10%, the TaqMan Cells-to-CT Express Kit can accommodate 45% of the total RT reaction volume as cell lysate. Additionally, cDNA can comprise up to 45% of the real-time PCR reaction volume. The large lysate volume in the optimized RT reaction, along with the large cDNA volume in the subsequent real-time PCR using the TaqMan Fast Advanced Master Mix, lead to maximum sensitivity. The master mix amplifies the target precisely and accurately, enabling the detection of small quantities of target, such as transcripts expressed at low levels.

Specifications

Specifications

• Express Lysis Solution (room temperature or 4°C)
• Express ezDNase (–20°C)
• SuperScript IV VILO Master Mix (–20°C)
• SuperScript IV VILO No RT Control (–20°C)
• TaqMan Fast Advanced Master Mix (4°C)

Liquid
High
Fast
Yes
ROX
cDNA
Direct cell lysis
Cultured mammalian cells
Primer-probe
Kit
2-step RT-qPCR
Less hazardous, less waste
Direct Cell Lysis RT-qPCR
Frozen, Dry Ice
Gene Expression
RNA
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only.