TaqMan™ Advanced miRNA Human Endogenous Controls 96-well Plate, fast

The Applied Biosystems TaqMan Advanced Human Endogenous Controls 96-Well Plate, Fast, provides the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well fast plate format.

Brand:  ABI Applied Biosystems A34643

Product Code. 17183089

Description

This product contains 1 plate than can be used to evaluate 30 potential endogenous control miRNAs for up to 3 samples. Two exogenous (non-human) controls are included for normalization of sample input.

Features of the TaqMan Advanced miRNA Human Endogenous Controls 96-Well Plate include:

• Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
• Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
• Updated content—assay content updated for mirBase v.21
• Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
• Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.

These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.

Specifications

• Content And Storage: Store at room temperature.
• Format: 96-well plate
• PCR Method: 2-step RT-qPCR
• For Use With (Equipment): 7500 Fast System, 7900HT Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, ViiA™ 7 System, StepOnePlus™ System
• Product Type: Endogenous Control 96-Well Plate
• For Use With (Application): Microarray Analysis
• Volume: 2 μL

• Detection Method: Primer-probe
• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Product Line: TaqMan™
• Shipping Condition: Room Temperature
• Sample Type: Liquid Samples (e.g. Serum), Tissue, Cells, FFPE & Fixed Samples, Blood

For Research Use Only. Not for use in diagnostic procedures.