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TaqMan™ Advanced miRNA Human B 96-well Plates, standard
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This product contains a set of 4 plates for profiling of up to 376 unique miRNAs, and includes endogenous and exogenous miRNA controls for normalization of data results.
Features of the TaqMan Advanced miRNA Human B 96-Well Plates include:
- Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
- Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
- Updated content—assay content updated for mirBase v.21
- Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
- Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families
TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
Specifications
Specifications
| Content And Storage | Store at room temperature. |
| Detection Method | Primer-probe |
| Format | 96-well plate |
| GC-Rich PCR Performance | High |
| PCR Method | 2-step RT-qPCR |
| Reaction Speed | Standard |
| For Use With (Equipment) | 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, ViiA™ 7 System, StepOnePlus™ System |
| Product Line | TaqMan™ |
| Product Type | Advanced miRNA Human 96-Well Plate |
| Quantity | 4 plates |
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For Research Use Only. Not for use in diagnostic procedures.
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