Applied Biosystems™ TaqMan™ Advanced miRNA Assay

Product Code. 15412184

Brand: Applied Biosystems™ A25576

Description

Applied Biosystems TaqMan Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR.

Applied Biosystems TaqMan Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR. Together with the TaqMan Advanced miRNA cDNA Synthesis Kit, this solution provides a streamlined upfront workflow that is ideal for analysis of multiple miRNA targets from a single sample or low-level RNA samples such as serum and plasma.

• Universal RT—one RT step for all TaqMan Advanced miRNA Assays
• Sensitivity—detect as few as 60 copies input into cDNA synthesis
• Specificity—detect only the mature miRNA and distinguish between highly homologous targets
• Small sample input—detect and quantify mature miRNA from as little as 1 pg total RNA or 2 μL plasma
• Compatibility with biofluids including human serum, plasma, and tissue

Pre-formulated assay

• 2 unlabeled PCR primers (900 nM each final 1X concentration)
• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

TaqMan miRNA Assay selection guide

TaqMan MicroRNA Assays

• Description: TaqMan MicroRNA Assays employ a novel target-specific stem–loop primer during cDNA synthesis to produce a template for real-time PCR
• RT chemistry: miRNA-specific RT
• Throughput: Best for 1–10 targets
• Coverage: 205 species available, coverage for miRBase v.21

TaqMan Advanced miRNA Assays

• Description: TaqMan Advanced miRNA Assays employ a universal RT step for a streamlined workflow, and a universal miR-Amp step to enable highly sensitive detection by real-time PCR
• RT chemistry: Universal RT
• Throughput: Best for > 10 targets
• Coverage: All human, mouse, and rat miRNAs; coverage for miRBase v.21

Order Info

Ambient Temperature

Specifications

• Concentration: 20X
• Content And Storage: 1 tube containing a 20X mix of pre-formulated assay (1 probe and 2 primers).
  
  Store at -15 to -25°C.
  
  
• Detection Method: Primer-probe
• Format: Tube
• GC-Rich PCR Performance: High
• PCR Method: qPCR
• Reaction Speed: Standard
• For Use With (Equipment): 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
• Product Line: TaqMan™
• Product Type: Advanced miRNA Assay
• Quantity: S (250 reactions), inventoried
• Shipping Condition: Room Temperature
• No. of Reactions: 250 Reactions
• Volume: 250 μL

For Research Use Only. Not for use in diagnostic procedures.