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Thermo Scientific™ Taq DNA Polymerase, recombinant (1 U/μL)

Optimize routine PCR with this highly thermostable DNA polymerase.

Brand:  Thermo Scientific™ EP0404

158.82 GBP valid until 2024-09-17
Use promo code "21690" to get your promotional price.



Code : 88

Additional Details : Weight : 1.51950kg

Product Code. 10180780

  • £177.00 / 500 units
Estimated Shipment: 19-07-2024
to see stock.

Description

Description

Optimize routine PCR with this highly thermostable DNA polymerase.

Thermo Scientific ™ Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.

PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Highlights

  • Thermostable -half life is more than 40 min at 95°C
  • Generates PCR products with 3'-dA overhangs
  • Supplied with two buffers -10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Specifications

Specifications

Contains:
• 500 μL Taq DNA Polymerase (1 U/μL)
• 2 x 1.25 mL 10X Taq Buffer with KCl
• 2 x 1.25 mL 10X Taq Buffer with (NH4)2SO4
• 2 x 1.25 mL 25 mM MgCl2

Store at -20°C.
Taq DNA Polymerase
Taq DNA Polymerase (recombinant), 1 U/μL
1 X
3'-A
Low
Standard
500 Units
No
Standalone
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For Research Use Only. Not for use in diagnostic procedures.