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Invitrogen™ TAP Tag Monoclonal Antibody (22F12-1E3)
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Product Code. 15226346
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Quantity:
50 μg
Unit Size:
50µg
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15226346 50 μg 50µg
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Product Code. 15226346 Supplier Invitrogen™ Supplier No. MA1108

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Description

Mouse Monoclonal Antibody

MA1-108 detects TAP in Western blot applications. The MA1-108 immunogen is a KLH-conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage. TAP adds about 20 kDa to the size of the protein.

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the TAP tag (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.
TRUSTED_SUSTAINABILITY

Specifications

Antigen TAP Tag
Applications Immunoprecipitation, Western Blot
Classification Monoclonal
Clone 22F12-1E3
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
Gene Alias Tobacco Etch Virus
Host Species Mouse
Immunogen KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage.
Purification Method Protein A
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Tag
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Antibody
Form Liquid
Isotype IgG1
Target Molecule Tag
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