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Thermo Scientific™ T4 DNA Ligase

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Brand:  Thermo Scientific™ EL0014

Code : 88

Additional Details : Weight : 0.01000kg

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Product Code. 10339509

  • £28.78 / 200 units


EU Stock 60
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Description

Description

Highlights

  • Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
  • Fast – sticky-end ligation is completed in 10 minutes at room temperature
  • Supplied with PEG solution for efficient blunt-end ligation

Applications

  • Cloning of restriction enzyme generated DNA fragments
  • Cloning of PCR products
  • Joining of double-stranded oligonucleotide linkers or adaptors to DNA
  • Site-directed mutagenesis
  • Amplified fragment length polymorphism (AFLP)
  • Ligase-mediated RNA detection (see Reference 3)
  • Nick repair in duplex DNA, RNA or DNA/RNA hybrids
  • Self-circularization of linear DNA.

Notes

  • Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.
  • The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.
  • Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture by spin column or chloroform extraction. The extracted DNA can be further precipitated with ethanol.
Specifications

Specifications

5U/μL
E.coli cells with a cloned gene 30 of bacteriophage T4
200U
T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration exceeds 200mM.
55.3 kDa monomer
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    2018-04-30

    *Discount will apply to qualifying orders received by Fisher Scientific between 01/01/18 and 30/04/2018. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

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