Invitrogen™ T-bet Monoclonal Antibody (eBio4B10 (4B10)), PerCP-Cyanine5.5, eBioscience™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15351190	Invitrogen™ 45-5825-80	25 μg
15361190	Invitrogen™ 45-5825-82	100 μg

Description

Description: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells. Applications Reported: This eBio4B10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eBio4B10 antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This antibody can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. For intracellular staining with the eBio4B10 monoclonal antibody...

T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development. T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression. Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.

Specifications

• Antigen: T-bet
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry
• Conjugate: PerCP-Cyanine5.5
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9JKD8, Q9UL17
• Gene ID (Entrez): 30009, 57765, 694044
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: eBio4B10 (4B10), 4-B10)
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1 κ
• Target Species: Human, Mouse, Rhesus Macaque
• Gene: Tbx21
• Gene Alias: HGNC:11599; LOW QUALITY PROTEIN: T-box transcription factor TBX21; Tbet; T-bet; TBLYM; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor 21; T-box transcription factor TBX21; TBT1; TBX21; T-cell-specific T-box transcription factor T-bet; T-PET; transcription factor TBLYM; transcription factor T-box 21
• Gene Symbols: Tbx21
• Product Type: Antibody