T-bet Monoclonal Antibody (eBio4B10 (4B10)), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5825-95

Product Code. 17168495

Description

Description: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells. Applications Reported: This eBio4B10 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections (with citrate antigen retrieval), immunoprecipitation, and immunoblotting (WB). Applications Tested: This eBio4B10 antibody has been tested by western blot analysis of Th1-polarized mouse CD4+ T cells and can be used at 2 μg/mL as a starting dilution for western blotting. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

T-bet Monoclonal antibody specifically detects T-bet in Human, Mouse, Rhesus Monkey samples. It is validated for Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot

Specifications

• Antigen: T-bet
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9JKD8, Q9UL17
• Host Species: Mouse
• Quantity: 10 mg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rhesus Monkey
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: eBio4B10 (4B10)
• Conjugate: Unconjugated
• Gene: Tbx21
• Gene Alias: HGNC:11599; TBET; T-bet; Tblym; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor 21; T-box transcription factor TBX21; TBT1; Tbx21; T-cell-specific T-box transcription factor T-bet; T-PET; Transcription factor TBLYM
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 30009, 57765, 694044
• Content And Storage: 4° C
• Form: Liquid