Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Artikelnummer.	Quantity	unitSize
15669364	1 L	Each
15679364	20 mL	Each
15659364	200 mL	Each
15689364	500 mL	Each
15626144	500 mL	500mL

Artikelnummer. 15669364 Lieferant Thermo Scientific™ Lieferanten-Nr. 34578

Beschreibung

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:

• Sensitivity: picogram to femtogram sensitivity
• Stability: 8-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 6 to 24 hours
• Recommended primary antibody concentration: 1:1000–1:5000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.

Spezifikation

• Product Type: Substrate
• Detection Method: Chemiluminescence
• Form: Liquid
• For Use With (Application): Western Blot
• Membrane Compatibility: Nitrocellulose, PVDF
• Product Line: SuperSignal
• Quantity: 1 L
• Conjugate: HRP
• Substrate: Horseradish Peroxidase
• Recommended Antibody Concentrations: 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
• Sensitivity: Picogram to high femtogram-level
• Signal Duration: 6 to 24 hr.
• Sufficient For: 120 Mini-Gel Blots, 10000 cm² of Membrane
• Kit Contents: Luminol/Enhancer, 500 mL, Stable Peroxide Buffer, 500 mL
• Content And Storage: Sufficient for 120 mini-gel blots; 10,000 cm² of membrane
  • SuperSignal West Pico PLUS Luminol/Enhancer, 500 mL
  • SuperSignal West Pico PLUS Stable Peroxide, 500 mL
  
  Store at room temperature.
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.