Thermo Scientific™ SuperSignal™ ELISA Femto Maximum Sensitivity Substrate

HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

Brand: Thermo Scientific™

Manufacturer Part Number: 37074

250 ML ELISA FEMTO CHEMILUMINESCENT SUBSTRATE,SuperSignal 250mL Store in Fridge at 4C Ship at

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: CAS Number: 18076-19-2 Weight: 0.25000kg

Product Code. 10023983

Quantity Price
1 £536.00 / 250mL
EU Stock 47
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Description and Specification


Description SuperSignal ELISA Femto Substrate
Formulation Proprietary luminol and peroxide solutions
Kit Contents Luminol/Enhancer, 125mL
Quantity 250mL kit
Sufficient For 250mL of 1X substrate (15 to 25 microplate assays)
Storage Requirements 4°C for up to one year or at room temperature for up to six months.

SuperSignal ELISA Femto Substrate generates detectable chemiluminescence (light) within one minute of addition to trace amounts of soluble HRP, saving up to 30 minutes per assay compared to colorimetric ELISA substrates. This feature is ideal for high-throughput screening (HTS) applications in which as many as 100,000 assays can be performed daily on robotic equipment. Because incubation periods are often a limiting factor in the development of rapid automated assays, SuperSignal ELISA Femto Substrate is the logical choice for automated HTS applications.


  • Immediate light generation – intense signal generated immediately at both room temperature and 37°C

  • Improved low-end linearity – easy detection of low quantities of proteins with high signal-to-noise ratios and low-end linearity of dose response curves

  • High sensitivity – femtogram-level detection of target proteins

  • Reduction in assay time – high sensitivity allows for reduction in incubation steps

  • Stability – storage for six months at room temperature or a minimum of 12 months at 4°C with a six-hour working solution stability

  • Emits light at 425nm – measurable by luminometers


Luminol/Enhancer Solution; Peroxide Buffer


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