Invitrogen™ SuperScript™ IV One-Step RT-PCR System

Product Code.	Includes	No. of Reactions	unitSize
15850233	Kit with ezDNase	100 Reactions	100 reactions
15814182	Kit only	25 Reactions	25 reactions
15781999	Kit only	100 Reactions	Each
15895891	Kit with ezDNase	25 Reactions	25 reactions

Product Code. 15850233 Supplier Invitrogen™ Supplier No. 12595100

Description

Includes

• SuperScript IV RT Mix, 12.5µL
• 2X Platinum SuperFi RT-PCR Master Mix, 625µL
• Nuclease-free water, 1.25mL

The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.

The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation plus gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube.

Features of the SuperScript IV One-Step RT-PCR System include:

• Superior performance with sensitivity down to 0.01 pg RNA, target length up to 13.8 kb, and the fastest one-step RT-PCR protocol
• Reliable target detection even with suboptimally pure RNA samples
• Two-phase hot-start activation mechanism for high sensitivity, specificity, and room temperature setup
• Fast and easy gDNA removal for superior accuracy and confidence in results (SuperScript IV One-Step RT-PCR System with ezDNase only)

SuperScript IV RT is a proprietary MMLV RT mutant with increased processivity, thermostability, and inhibitor resistance, as well as reduced RNase H activity, for sensitive and efficient full-length cDNA synthesis. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. The combination of these two engineered enzymes produces enhanced RT-PCR yields and fidelity, as well as amplification of longer templates compared to other one-step RT-PCR kits.

The inclusion of ezDNase (SuperScript IV One-Step RT-PCR System with ezDNase only) for use upstream of the RT step further enhances the one-step RT-PCR workflow through an extremely simplified genomic DNA removal step that ensures the highest accuracy and confidence in one-step RT-PCR results.

Specifications

• Content And Storage:

  • SuperScript IV RT Mix (50 μL)
  • 2X Platinum SuperFi RT-PCR Master Mix (2 x 1.25 mL)
  • ezDNase Enzyme (100 μL)
  • 10X ezDNase Buffer (100 μL)
  • Nuclease-free water (2 x 1.25 mL)
  
  Store at –20°C.
  
  

• Detection Method: Gel Electrophoresis
• Format: Kit
• GC-Rich PCR Performance: High
• PCR Method: 1-step RT-PCR
• Polymerase: Platinum SuperFi
• Reaction Speed: 10 min.
• Technique: 1-step RT-PCR
• Optimal Reaction Temperature: 45°C to 60°C
• Quantity: 1 Ea.
• Reverse Transcriptase: SuperScript IV
• Ribonuclease H Activity: Reduced
• Shipping Condition: Dry Ice
• Final Product Type: PCR Amplified cDNA
• Hot Start: Built-In Hot Start
• No. of Reactions: 100 Reactions
• Reaction Format: Master Mix
• Reagent Type: Reverse Transcription
• Size (Final Product): Up to 14 kb
• Starting Material: RNA
• Includes: Kit with ezDNase

For Research Use Only. Not for use in diagnostic procedures.