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STEMCELL Technologies STEMdiff™ Monocyte Kit
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Quantity:
1 Kit
Unit Size:
1 set
Description
- STEMdiff™ Monocyte Kit includes serum-free media and supplements for the feeder-free differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells to monocytes expressing CD14
- The simple protocol is performed in 2D adherent cultures
- During the first 3 days, Medium A induces cells toward mesoderm
- For the subsequent 4 days, mesodermal cells are further differentiated toward the hematopoietic lineage using Medium B
- At Day 7, the medium is changed to Monocyte Differentiation Medium, which facilitates the differentiation to monocytes
- CD14+ monocytes can be harvested directly from the culture supernatant starting as early as Day 14 and can be repeatedly harvested during the rest of the culture period
- Peak CD14+ frequency is typically 60 - 80%
Specifications
Specifications
| Cell Type | Dendritic Cells, Macrophages, Monocytes, Myeloid Cells, Pluripotent Stem Cells |
| For Use With (Application) | Cell Culture, Differentiation, Expansion |
| Quantity | 1 Kit |
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