Invitrogen™ STAT Family Activation Human InstantOne™ ELISA Kit

Product Code. 15520797

Brand: Invitrogen™ 858609511

Description

Includes

• User Manual
• InstantOne™ Assay Plate - 96 well (sealed)
• Cell Lysis Buffer (5X)
• Enhancer Solution
• Wash Buffer (10X)
• phospho-STAT1 (Tyr701) Capture Antibody Reagent
• phospho-STAT3 (Tyr705) Capture Antibody Reagent
• phospho-STAT5A/B (Tyr694/699) Capture Antibody Reagent
• phospho-STAT1 (Tyr701) Detection Antibody Reagent
• phospho-STAT3 (Tyr705) Detection Antibody Reagent
• phospho-STAT5A/B (Tyr694/699) Detection Antibody Reagent
• Positive Control Cell Lysate (lyophilized)
• Detection Reagent (TMB Substrate)
• Stop Solution
• Plate Seal

InstantOne ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human STAT1 (Tyr701), STAT3 (Tyr705), and STAT5A/B (Tyr694/699) in cell lysates. Detection of mouse and rat phosphorylated STAT5A/B is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.

Specifications

• Accession Number: P40763,P42224,P42229,P51692
• Conjugate: Biotin
• Product Type: ELISA Kit
• Sample Type: Cell Lysates
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 6772, 6774, 6776, 6777
• Gene Symbol: ADMIO,ADMIO1,APRF,HIES,CANDF7,IMD31A,IMD31B,IMD31C,ISGF-3,STAT91,MGF,STAT5
• Kit Contents: Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate,20 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Signal Transducer and Activator of Transcription
• Target: Human
• Test Time: 1 hr. 20 min.
• Total Assay Time: 1 hr. 10 min.

For Research Use Only