Thermo Scientific™ StartingBlock™ Blocking Buffer

Product Code.	Quantity	Chemical Name or Material	Unit Size
10270404	1 L	PBST	1 L
13414299	100 mL	PBS	100mL
10108313	1 L	PBS	1 L
13424299	100 mL	TBS	100mL
30089296	3 x 1 L	PBS	Pack of 3
10371804	1 L	TBS	1 L
10381804	1 L	TBST	1 L

Código de producto. 10270404 Proveedor Thermo Scientific™ N.º de proveedor 37539

Descripción

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays.

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays. It provides broad compatibility with a wide range of antibodies, antibody combinations, and other protein probing and assay systems. StartingBlock Blocking Buffer is available in PBS and TBS, with and without Tween 20.

Features of StartingBlock Blocking Buffer include:

• Strip and re-probe without re-blocking—blots stay blocked even after stripping with Restore Stripping Buffer (Cat. No. 21059)
• Compatible with many detection systems—western blot, ELISA, and IHC with antibody or avidin/biotin probes (blocker is serum- and biotin-free)
• Short blocking times—less than 15 minutes for nitrocellulose or PVDF membranes; almost instantaneous for polystyrene microplate wells

Especificaciones

• Chemical Name or Material: PBST
• Concentration: 1X
• For Use With (Application): Western Blot
• Physical Form: Liquid
• Product Line: StartingBlock
• Quantity: 1 L
• Formulation: Proprietary protein formulation in phosphate-buffered saline, pH 7.5, with 0.05% Tween 20

Frequently Asked Questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.