IBA Solutions For Life Science StarGate™ Upstream Fusion Vector pNFUSE-IBA

Product Code. 15693718

Brand: IBA Solutions For Life Science 51631001

Description

For upstream positioning of an inserted gene in the final Fusion Donor vector when linking two or more genes cloned in separate Donor Vectors using the StarGate™ transfer cloning system.

StarGate™ offers a simple cloning procedure for rapid and highly efficient subcloning of an arbitrary gene of interest (GOI) for use in functional expression, purification, detection, and immobilization or separation of recombinant proteins.

• Minimal extra modification of the gene due to short combinatorial sites
• Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
• Genes are cloned in a Donor Vector and then parallel-transfered into Acceptor Vectors that provide different genetic surroundings
• Easily connect two or more genes that are cloned in separate Donor Vectors using Fusion Vectors that are assembled by use of a one-tube reaction
• The GOI to be positioned upstream is transferred into a pNFUSE-IBA derivative, specifying the intergenic region (IR):
  • IRES11: Provides internal ribosomal entry site (IRES) from encephalomyocarditis virus for separate cap-independent translation of downstream cistron in mammalian cells
  • SD11: IR includes a Shine Dalgarno (SD) sequence for translation of downstream cistron at comparable level to the upstream cistron in bacterial cells
  • LINK11: Genes of interest are fused by a sequence encoding the 10 amino acid linker GlySerGlyGlyGlySerGlyGlyGlySer
  • LINK12: Genes of interest are fused by a sequence encoding the 15 amino acid linker GlySerGlyGlySerGlyGlyGlyGlySerGlyGlyGlyGlySer
• The GOIs to be positioned downstream are transferred into a pCFUSE-IBA11 vector (identical, irrespective of IR)

Specifications

• Format: Solution, liquid
• For Use With (Equipment): Stargate™ system derivatives
• Description: Resistance: ampicillin
• Includes: IR: Internal ribosomal entry site (IRES) from encephalomyocarditis virus
• pH: 8
• Product Type: Fusion Vector
• Storage Buffer: 20μL TE buffer: 10mM Tris-HCl, 1mM EDTA per 5μL pCFUSE-IBA11
• Content And Storage: 2°C to 8°C for frequent usage, -20°C for long-term storage
• Concentration: 250 ng/μL
• Quantity: 5 μg
• Vector: pNFUSE-IBA w/ IRES11
• Color: Clear
• For Use With (Application): Upstream positioning, fusing cloned genes of interest