IBA Lifesciences StarGate pLSG-IBA Vectors for Baculo

Transfer vectors that allow for high-level expression in insect cells by introducing the gene of interest into the Polyhedrin gene locus of AcMNPV DNA by homologous recombination.

Product Code	Brand	Description	Protein Tag	Includes
15623828	IBA Lifesciences 5-4800-001	pLSG-IBA wt1	Untagged	pUC ori, Ampicillin resistance
15633828	IBA Lifesciences 5-4801-001	pLSG-IBA wt2	Untagged	BM40 signal, pUC ori, Ampicillin resistance
15643828	IBA Lifesciences 5-4803-001	pLSG-IBA 3	Strep-tag for C-terminal	pUC ori, Ampicillin resistance
15653828	IBA Lifesciences 5-4805-001	pLSG-IBA 5	Strep-tag for N-terminal	pUC ori, Ampicillin resistance
15663828	IBA Lifesciences 5-4823-001	pLSG-IBA 23	Strep-tag for C-terminal and GST-tag for N-terminal	pUC ori, Ampicillin resistance
15603838	IBA Lifesciences 5-4843-001	pLSG-IBA 43	Strep-tag for C-terminal and His Tag (6x) for N-terminal	pUC ori, Ampicillin resistance
15613838	IBA Lifesciences 5-4845-001	pLSG-IBA 45	Strep-tag for N-terminal and His Tag (6x) for C-terminal	pUC ori, Ampicillin resistance
15623838	IBA Lifesciences 5-4862-001	pLSG-IBA 62	Strep-tag for C-terminal and FLAG-tag for N-terminal	pUC ori, Ampicillin resistance
15643838	IBA Lifesciences 5-4864-001	pLSG-IBA 64	Strep-tag for N-terminal and FLAG-tag for C-terminal	pUC ori, Ampicillin resistance
15663838	IBA Lifesciences 5-4902-001	pLSG-IBA 102	Twin-Strep-tag for C-terminal	BM40 signal, pUC ori, Ampicillin resistance
15673838	IBA Lifesciences 5-4903-001	pLSG-IBA 103	Twin-Strep-tag for C-terminal	pUC ori, Ampicillin resistance
15683838	IBA Lifesciences 5-4904-001	pLSG-IBA 104	Twin-Strep-tag for N-terminal	BM40 signal, pUC ori, Ampicillin resistance
15693838	IBA Lifesciences 5-4905-001	pLSG-IBA 105	Twin-Strep-tag for N-terminal	pUC ori, Ampicillin resistance
15603848	IBA Lifesciences 5-4923-001	pLSG-IBA 123	Twin-Strep-tag for C-terminal and GST-tag for N-terminal	pUC ori, Ampicillin resistance
15613848	IBA Lifesciences 5-4942-001	pLSG-IBA 142	Twin-Strep-tag for C-terminal and His Tag (6x) for N-terminal	BM40 signal, pUC ori, Ampicillin resistance
15623848	IBA Lifesciences 5-4943-001	pLSG-IBA 143	Twin-Strep-tag for C-terminal and His Tag (6x) for N-terminal	pUC ori, Ampicillin resistance
15633848	IBA Lifesciences 5-4944-001	pLSG-IBA 144	Twin-Strep-tag for N-terminal and His Tag (6x) for C-terminal	BM40 signal, pUC ori, Ampicillin resistance
15643848	IBA Lifesciences 5-4945-001	pLSG-IBA 145	Twin-Strep-tag for N-terminal and His Tag (6x) for C-terminal	pUC ori, Ampicillin resistance
15653848	IBA Lifesciences 5-4962-001	pLSG-IBA 162	Twin-Strep-tag for C-terminal and FLAG-tag for N-terminal	pUC ori, Ampicillin resistance
15663848	IBA Lifesciences 5-4964-001	pLSG-IBA 164	Twin-Strep-tag for N-terminal and FLAG-tag for C-terminal	pUC ori, Ampicillin resistance
15673848	IBA Lifesciences 5-4967-001	pLSG-IBA 167	Twin-Strep-tag/FLAG-tag for N-terminal	pUC ori, Ampicillin resistance
15683848	IBA Lifesciences 5-4968-001	pLSG-IBA 168	Twin-Strep-tag/FLAG-tag for C-terminal	pUC ori, Ampicillin resistance
15673828	IBA Lifesciences 5-4825-001	pLSG-IBA 25	GST Tag for N-terminal	pUC ori, Ampicillin resistance
15653838	IBA Lifesciences 5-4865-001	pLSG-IBA 65	FLAG-tag for N-terminal	pUC ori, Ampicillin resistance
15683828	IBA Lifesciences 5-4833-001	pLSG-IBA 33	His Tag (6x) for C-terminal	pUC ori, Ampicillin resistance
15693828	IBA Lifesciences 5-4835-001	pLSG-IBA 35	His Tag (6x) for N-terminal	pUC ori, Ampicillin resistance
15633838	IBA Lifesciences 5-4863-001	pLSG-IBA 63	FLAG-tag for C-terminal	pUC ori, Ampicillin resistance

Description

pLSG-IBA Baculo virus (insect cells) expression vectors

The pLSG-IBAwt1 vector carries the Polyhedrin promoter for high-level expression in insect cells.

• Co-transfection with BacPAK6 linearized AcMNPV DNA (Clontech) or circular flashBAC modified AcMNPV DNA (Oxford Expression Technologies) allows the generation of recombinant baculovirus at very high efficiency through reconstitution of an essential gene (ORF 1629) and elimination of wild type virus to great extent.
• Cloning and expression of recombinant proteins in insect cells
• Polyhedrin promoter drives high-level expression of the insert
• Ampicillin resistance and ColE1 origin of replication (pUC) support propagation in E. coli

Please note that cloning into pLSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pLSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

• Format: Liquid
• Inducing Agent: BacPAK6 linearized A cmNPV DNA (Clontech) or circular flashBAC modified A cmNPV DNA (Oxford Expression Technologies)
• Promoter: Polyhedrin
• Storage Buffer: 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
• Concentration: 250 ng/μL
• Color: Clear

• For Use With (Equipment): StarGate™ System, Insect Host Cells
• pH: 8
• Product Type: Acceptor Vector for bacula (insect cell) expression
• Content And Storage: 2°C to 8°C for frequent usage, -20°C for long-term storage
• Quantity: 5 μg
• For Use With (Application): Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins