Invitrogen™ Staphylococcus aureus Cas9 Monoclonal Antibody (6H4), eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen™ 14-6499-82

Product Code. 17170682

Description

Description: This 6H4 monoclonal antibody recognizes Staphylococcus aureus Cas9 endonuclease. Applications Reported: This 6H4 antibody has been reported for use in immunocytochemical staining and western blot. (Fluorochrome-conjugated 6H4 is recommended for use in flow cytometery.) Applications Tested: This 6H4 antibody has been tested by immunocytochemical staining of Staphylococcus aureus derived Cas9 transfected A549 cells. This may be used at less than or equal to 0.6 μg/mL. This 6H4 antibody has been tested by western blot of Staphylococcus aureus derived Cas9 transfected A549 cells. This may be used at less than or equal to 4 μg/mL detecting a band at 124 kDa.

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.

Specifications

• Antigen: Staphylococcus aureus Cas9
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Bacteria
• Product Type: Antibody
• Isotype: IgG2b κ

• Applications: Western Blot, Immunocytochemistry
• Clone: 6H4
• Conjugate: Unconjugated
• Gene Alias: cas9; CRISPR9; CRISPR-Cas9; s aureus
• Immunogen: His-tagged recombinant Cas-9 from Staphylococcus aureus (maps to N-terminus).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4°C, do not freeze
• Form: Liquid