SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-8843-95

Product Code. 17158585

Description

Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells. The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys. Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry. Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been tested by flow cytometric analysis of the 2102Ep cell line. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

SSEA4 Monoclonal antibody specifically detects SSEA4 in Canine, Chicken, Human, Mouse, Non-human Primate, Rabbit samples. It is validated for Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Specifications

• Antigen: SSEA4
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Host Species: Mouse
• Quantity: 10 mg
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Form: Liquid

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Clone: eBioMC-813-70 (MC-813-70)
• Conjugate: Unconjugated
• Gene Alias: SSEA-4; stage-specific embryonic antigen 4
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Canine, Chicken, Human, Mouse, Non-human Primate, Rabbit
• Product Type: Antibody
• Isotype: IgG3