SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15550257	Affymetrix eBioscience 53-8833-41	25 Tests
15560257	Affymetrix eBioscience 53-8833-42	100 Tests

Description

Description: The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) and down regulated following differentiation. Applications Reported: This eBioMC-631 (MC-631) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioMC-631 (MC-631) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

Specifications

• Antigen: SSEA3
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Isotype: IgM
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: eBioMC-631 (MC-631)
• Applications: Flow Cytometry
• Conjugate: Alexa Fluor 488
• Host Species: Rat
• Target Species: Human, Mouse
• Gene Alias: SSEA-3; stage-specific embryonic antigen 3
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

For Research Use Only.