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SSEA4, eFluor 660, clone: eBioMC-813-70 (MC-813-70), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-8843-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15530057

  • £172.54 / 100µg


Estimated Shipment date
from Supplier 30-05-2018
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Description

Description

Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells.The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys.Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis, and immunocytochemistry.Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been tested by flow cytometric analyis or immunocytochemistry. For flow cytometry, this can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For ICC, this can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

SSEA-4
Monoclonal
0.2mg/mL
PBS with 0.09% sodium azide; pH 7.2
stage-specific embryonic antigen 4, SSEA-4
IgG3
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
Rabbit
Immunocytochemistry
eBioMC-813-70 (MC-813-70)
eFluor 660
Conjugated
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
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