SSEA-1 Mouse, PE, Clone: MC480, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 560886

Product Code. 15830569

Description

The MC480 monoclonal antibody reacts with Stage-Specific Embryonic A ntigen-1 (SSEA-1), which is a terminal carbohydrate epitope (3-fucosyl-N-acetyllactosamine or 3-FAL) on glycoproteins and lactose series glycolipids. SSEA-1 is related to Lewis blood group antigens and is found in a variety of embryonic and adult tissues and cancers. As its name implies, the expression of SSEA-1 is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. However, its expression pattern differs in the human and mouse. In the human, SSEA-1 is not found on embryonic stem (ES) cells, embryonic inner cell mass (ICM), or teratocarcinoma (embryonal carcinoma or EC) cells. As human EC and ES cells undergo differentiation, SSEA-1 expression is upregulated. In the adult, the same epitope is expressed as CD15 on granulocytes and monocytes, but not lymphocytes or dendritic cells. In the mouse, SSEA-1 is found on EC, ES, and primordial germ cells, 8-cell to blastocyst embryos, ICM, and on subpopulations of cells in the adult central nervous system, including stem cells. In contrast to the human, SSEA-1 expression is reduced as mouse EC and ES cells undergo differentiation.

Specifications

• Antigen: SSEA-1
• Classification: Monoclonal
• Conjugate: PE
• Formulation: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
• Immunogen: Mouse Teratocarcinoma Cell Line
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: MC480
• Description: 3-FAL, X-hapten, LeX antigen, CD15
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse
• Isotype: IgM κ

For Research Use Only.