UNG Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 4 publications

Product Code	Brand	Quantity
18237732	Novus Biologicals NB600-1031SS	0.025 mg
18750224	Novus Biologicals NB600-1031	0.1 mg

Description

UNG Polyclonal specifically detects UNG in Human, Mouse, Rat, Rabbit samples. It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: UNG
• Dilution: Western Blot 2 ug/ml, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500. Use reported in scientific literature (Yang et al (2005)), Immunohistochemistry-Paraffin 10 ug/ml
• Classification: Polyclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Rabbit
• Gene Accession No.: NP_550433
• Gene ID (Entrez): 7374
• Immunogen: This antibody was generated by immunizing rabbit with a synthetic peptide sequence CRHFSKTNELLQKSGKKP corresponding to amino acids 281-298 of human UNG1 (NP 003353.1) and amino acids 290-307 of human UNG2 (NP 550433.1). The peptide sequence used for immunogen is highly conserved between mammalian and invertabrates.
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Concentration: 1.0 mg/ml
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Rabbit
• Research Discipline: Base Excision Repair, DNA Repair, Stem Cell Markers
• Formulation: PBS with 0.05% Sodium Azide
• Gene Alias: DGUEC 3.2.2, HIGM4EC 3.2.2.27, UDGDKFZp781L1143, UNG15, UNG1uracil-DNA glycosylase 1, uracil-DNA glycosylase 2, UNG2, uracil-DNA glycosylase
• Gene Symbols: UNG
• Isotype: IgG
• Purification Method: Protein G purified
• Test Specificity: The synthetic peptide sequence used for immunogen is 100% conserved between UNG1 and UNG2. Thus the antibody recognizes both UNG1 and UNG2. As UNG1 and UNG2 are not resolved as two species in gel electrophoresis (Huag et al., 1998), they are indistinguishable by western blot. The observed molecular weight of UNG (UNG1 and UNG2) on western blots may vary: 26 kDa, 27.5 kDa, 31 kDa, and 38 kDa forms have been described (Bharati et al., 1998; Slupphaug et al., 1993). Please see Kruman et al. (Fig. 1D, 2004) for an example of western blot in rat. This specificity of this antibody has been validated by antisense UNG oligonucleotide (Kruman et al. 2004).

For Research Use Only