Invitrogen™ SPT5 Polyclonal Antibody

Product Code.	Quantity	unitSize
17742624	100 μL	100µL

Product Code. 17742624 Supplier Invitrogen™ Supplier No. PA5121226

Description

Rabbit Polyclonal Antibody

Positive test controls include: U-87MG, HeLa, A-549, Mouse liver. The target is usually found in the following locations: Nucleus. Immunogen sequence: LQNLWRDQRE EELGEYYMKK YAKSSVGETV YGGSDELSDD ITQQQLLPGV KDPNLWTVKC KIGEERATAI SLMRKFIAYQ FTDTPLQIKS VVAPEHVKGY IYVEAYKQTH VKQAIEGVGN LRLGYWNQQM VPIKEMTDVL KVVKEVANLK PKSWVRLKRG IYKDDIAQVD YVEPSQNTIS LKMIPRIDYD RIKARMSLKD WFAKRKKFKR PPQRLFDAEK IRSLGGDVAS DGDFLIFEGN RYSRKGFLFK SFAMSAVITE GVKPTLSELE KFEDQPEGID LEVVTESTGK EREHNFQPGD N.

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. [UniProt].

Specifications

• Antigen: SPT5
• Applications: ELISA, Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Concentration: 2.27 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene: SUPT5H
• Gene Accession No.: O00267, O55201
• Gene Alias: AL033283; AL033321; AU019549; DRB sensitivity-inducing factor 160 kDa subunit; DRB sensitivity-inducing factor large subunit; DSIF large subunit; DSIF p160; etID10129.15; etID37101.15; fb37g01; fb95e05; fog; foggy; hSPT5; protein foggy; Spt5; SPT5 homolog, DSIF elongation factor subunit; SPT5H; suppressor of Ty 5; suppressor of Ty 5 homolog; suppressor of Ty 5 homolog (S. cerevisiae); Supt5; Supt5h; supt5h protein; Tat-cotransactivator 1 protein; Tat-CT1; Tat-CT1 protein; Transcription elongation factor SPT5; transcription elongation regulator FOGGY; wu:fb37g01; wu:fb95e05; wu:fc10f02
• Gene Symbols: Supt5, SUPT5H
• Host Species: Rabbit
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 125-425 of human SUPT5H/SPT5 (NP_003160.2).
• Purification Method: Affinity Chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 20924, 6829
• Target Species: Human, Mouse
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG