Invitrogen™ SPIN2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA576218

Product Code. 15942745

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Human SPIN2 expressed by 2 IL3-dependent murine myeloid cell lines was moderately antiapoptotic following IL3 removal, but it was unable to block apoptosis induced by the chemotherapy agent doxorubicin. On the contrary, SPIN2-expressing cells were more sensitive to doxorubicin-mediated cell death. Also, SPIN2 did not combat the proapoptotic effects of Fas ligand stimulation in the Jurkat human T-cell line. Deletion of the C-terminal amino acids of SPIN2 diminished its antiapoptotic activity. In the murine myeloid cells, SPIN2 overexpression increased the cell number doubling times and slowed their rate of growth. There was also an increased percentage of cells in G2/M, which was more pronounced following IL3 withdrawal.

Specifications

• Antigen: SPIN2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SPIN2B
• Gene Alias: BC068162; dJ323P24.2; Spin2; SPIN-2; SPIN2_duplicate; SPIN2B; SPIN-2B; Spin2c; SPIN-2C; Spindlin 2B; spindlin family member 2B; spindlin family, member 2; spindlin family, member 2 duplicate; spindlin family, member 2B; spindlin family, member 2C; spindlin-2; spindlin-2B; Spindlin-2C; spindlin-like protein 2; spindlin-like protein 2B; Spindlin-like protein 2C; TDRD26; telomeric SPIN2 copy
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 278240, 474343
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q6NVE3, Q9BPZ2
• Gene Symbols: SPIN2B, Spin2c
• Immunogen: Recombinant protein of human SPIN2B.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG