Invitrogen™ SOX9 Recombinant Mouse Monoclonal Antibody (GMPR9), Alexa Fluor™ Plus 488

Mouse Recombinant Monoclonal Antibody

Product Code	Brand	Quantity
30276414	Invitrogen™ 740051MP488	50 μL
30276530	Invitrogen™ 740051MP48820UG	20 μL

Beschreibung

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Applications Tested: This GMPR9 antibody has been tested by immunocytochemistry and flow cytometric analysis of PC-3 cells. This may be used for immunocytochemistry at 5 μg/mL and for flow cytometry at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 4...

SOX9 has a role in sex determination and differentiation of Sertoli cells. It is involved in chondrogenesis and regulates the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Translocation of this gene can cause campomelic dysplasia. SOX9 is involved in the formation of testes from the indifferent fetal gonads. It is a major molecular component of the neuron-glia switch in developing spinal cord. During normal development, SOX9 allows the prostate epithelium to outgrow into the mesenchyme and then provides basal cell support for development and maintenance of the luminal epithelium. These functions of SOX9 are subverted in prostate cancer to support tumor growth and invasion. SOX9 may direct the formation of neural crest precursors and the development of a range of neural crest derivative. It has a transcriptional regulation in melanin production in cells. Northern blot analysis shows its expression on adult testis, adult heart, and fetal brain.

Spezifikation

• Antigen: SOX9
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Conjugate: Alexa Fluor Plus 488
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8
• Gene Accession No.: P48436, Q04887
• Gene ID (Entrez): 140586, 20682, 6662
• Primary or Secondary: Primary
• Purification Method: Protein A/G

• Clone: GMPR9
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Recombinant Monoclonal
• Form: Liquid
• Isotype: IgG1 κ
• Target Species: Human, Mouse, Rat
• Gene: SOX9
• Gene Alias: 2010306G03Rik; AV220920; CMD1; CMPD1; mKIAA4243; SOX 9; Sox9; SOX-9; Sox9 transcription factor; SRA1; SRXX2; SRXY10; SRY (sex determining region Y)-box 9; SRY (sex determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal); SRY (sex determining region Y)-box9; SRY (sex-determining region Y)-box 9 protein; SRY box 9; SRY-box 9; SRY-Box 9 ; SRY-box containing 9; SRY-box containing gene 9; SRY-box containing protein 9; SRY-box transcription factor 9; SRY-related HMG-box, gene 9; transcription activator Sox9; transcription factor SOX-9
• Gene Symbols: SOX9
• Product Type: Antibody