SOX10 Mouse anti-Human, Mouse, Clone: SOX10/991, Novus Biologicals™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
18208634	Novus Biologicals NBP2-59620-20UG	20 μg
18246833	Novus Biologicals NBP2-59620-100UG	100 μg

Description

SOX10 Monoclonal specifically detects SOX10 in Human, Mouse samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Flow (Intracellular).

Specifications

• Antigen: SOX10
• Concentration: 0.2 mg/mL
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), SDS-Page
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Mouse
• Gene Alias: MGC15649, PCWH, SRY (sex determining region Y)-box 10, SRY-related HMG-box gene 10, transcription factor SOX-10, WS4C, WS4mouse, human homolog of
• Gene Symbols: SOX10
• Isotype: IgG2b κ
• Purification Method: Protein A or G purified
• Test Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of ∼55kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG -box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells

• Clone: SOX10/991
• Dilution: Western Blot 0.5-1 ug/ml, Flow Cytometry 0.5-1 ug/million cells, Immunocytochemistry/Immunofluorescence 1-2 ug/ml, Immunohistochemistry-Paraffin 0.5-1 ug/ml, SDS-Page, Protein Array 1:100-1:2000
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Formulation: 10mM PBS with 0.05% BSA with 0.05% Sodium Azide
• Gene ID (Entrez): 6663
• Immunogen: Recombinant human SOX10 protein fragment (around aa115-269) (exact sequence is proprietar
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 54 kDa

For Research Use Only