Invitrogen™ Smoothelin Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA562481

Product Code. 15780272

Description

Immunogen sequence: LAGLDEGALR KLLEVTADLA ERRRIRSAIR ELQRQELERE EEALASKRFR AERQDNKENW LHSQQREAEQ RAALARLAGQ LESMNDVEEL TALLR Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 96%.

Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are a useful tool to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses reveal a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts, myoepithelial cells, and skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin and vimentin. Smoothelin colocalizes with actin stress fibers. Two tissue specific isoforms have been identified: a 59 kDa isoform specific for visceral SMC; and a 100 kDa specific for vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22.

Specifications

• Antigen: Smoothelin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SMTN
• Gene Alias: FLJ35365; FLJ38597; smoothelin; smoothelin-B; smsmo; SMTN
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6525
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.2 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: P53814
• Gene Symbols: SMTN
• Immunogen: Recombinant protein corresponding to Human Smoothelin. Recombinant protein control fragment (Product #RP-103107).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG