Invitrogen™ SMCR8 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA554449

Product Code. 15752381

Description

Immunogen sequence: SQASTTSNPD ESADTDLYTC RPAYTPKLIK AKSTKCFDKK LKTLEELCDT EYFTQTLAQL SHIEHMFRGD LCYLLTSQID RALLKQQHIT NFLFEDFVEV DDRMVEKQES IPSKPSQD Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 86%.

Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy. In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation. The C9orf72-SMCR8 complex also acts as a negative regulator of autophagy initiation by interacting with the ATG1/ULK1 kinase complex and inhibiting its protein kinase activity. Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates. In addition to its activity in the cytoplasm within the C9orf72-SMCR8 complex, SMCR8 also localizes in the nucleus, where it associates with chromatin and negatively regulates expression of suppresses ULK1 and WIPI2 genes.

Specifications

• Antigen: SMCR8
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SMCR8
• Gene Alias: 2310076G09Rik; AI642055; D030073L15Rik; FLJ34716; Guanine nucleotide exchange protein SMCR8; RGD1564621; SMCR8; Smith-Magenis syndrome chromosomal region candidate gene 8 protein; Smith-Magenis syndrome chromosomal region candidate gene 8 protein homolog; Smith-Magenis syndrome chromosome region, candidate 8; Smith-Magenis syndrome chromosome region, candidate 8 homolog; Smith-Magenis syndrome chromosome region, candidate 8 homolog (human)
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 140775
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunocytochemistry
• Concentration: 0.2 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q8TEV9
• Gene Symbols: SMCR8
• Immunogen: Recombinant protein corresponding to Human SMCR8. Recombinant protein control fragment (Product #RP-93456).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG