Smad3, p Ser423, p Ser425 Antibody, Alexa Fluor™ 750, Novus Biologicals™

Product Code.	Quantity	unitSize
18366391	0.1 mL	0.1mL

Produktkod. 18366391 Leverantör Novus Biologicals Leverantörsnummer NBP177836AF750

Beskrivning

Rabbit Polyclonal Antibody

Smad3 Polyclonal antibody specifically detects Smad3 in Human, Mouse samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin).

Specifikationer

• Antigen: Smad3
• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
• Classification: Polyclonal
• Conjugate: Alexa Fluor 750
• Dilution: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Alias: DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
• Gene Symbols: SMAD3
• Host Species: Rabbit
• Immunogen: Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022)
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Gene ID (Entrez): 4088
• Test Specificity: This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus species laevis, Xenopus species tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
• Target Species: Human, Mouse, Rat, Pig, Bovine, Chicken, Xenopus species, Zebrafish
• Content And Storage: Store at 4°C in the dark.
• Isotype: IgG