Invitrogen™ SLP76 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA596165

Product Code. 16589001

Description

Positive Samples: U-937, BT-474, Mouse spleen, Mouse liver; Cellular Location: Cytoplasm Immunogen sequence: SILPAKPFPN SNSMYIDRPP SGKTPQQPPV PPQRPMAALP PPPAGRNHSP LPPPQTNHEE PSRSRNHKTA KLPAPSIDRS TKPPLDRSLA PFDREPFTLG KKPPFSDKPS IPAGRSLGEH LPKIQKPPLP PTTERHERSS PLPGKKPPVP KHGWGPDRRE NDEDDVHQRP LPQPALLPMS SNTFPSRSTK PSPMNPLPSS HMPGAFSESN SSFPQSASLP PYFSQGPSNR PPIRAEGRNF PLPLPNKPRP PSPAEEENSL NEEWYVSYIT RPEAEAALRK INQDGTFLVR DSSKKTTTNP YVLMVLYKDK VYNIQIRYQK ESQVYLLGTG LRGKEDFLSV SDIIDYFRKM PLLLIDGKNR GSRYQCTLTH AAGYP.

SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.

Specifications

• Antigen: SLP76
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: LCP2
• Gene Alias: 76 kDa tyrosine phosphoprotein; AI323664; BB161688; Lcp2; Lymphocyte cytosolic protein 2; lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa); lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of 76kD); SH2 domain-containing leukocyte protein of 76 kDa; SH2 domain-containing leukocyte protein of 76kD; SLP76; SLP-76; SLP-76 tyrosine phosphoprotein; twm
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16822, 3937
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.64 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: Q13094, Q60787
• Gene Symbols: LCP2
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 159-533 of human LCP2 (NP_0055561).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG