Thermo Scientific™ Slide-A-Lyzer™ Accessory Float Buoys and Syringes

Artikelnummer.	Capacity (Metric)	Product Type	unitSize
10770995	For 0.5 to 3 mL Cassettes	Cassette Float Buoy	Pack of 10
10197393	For 12 mL Cassettes	Cassette Float Buoy	Pack of 8
10733495	5 mL	Cassette Syringe	Pack of 10
10187493	20 mL	Cassette Syringe	Pack of 10
10570904	1 mL	Cassette Syringe	Pack of 10

Artikelnummer. 10770995 Leverandør Thermo Scientific™ Leverandørnr. 66430

Beskrivelse

Slide-A-Lyzer Float Buoys and Syringes are available to support a variety of dialysis cassettes for volumes of 0.1 to 20 mL.

Slide-A-Lyzer Dialysis Cassette Syringes are designed for efficient loading of samples into Slide-A-Lyzer cassettes and removal of sample after dialysis. Cassette Float Buoy accessories are for use with Slide-A-Lyzer Dialysis Cassettes used to remove low molecular weight contaminants and perform buffer exchange, desalting, and concentration.

Minimize sample loss into and out of Slide-A-Lyzer Dialysis Cassettes using Slide-A-Lyzer Dialysis Cassette Syringes for 1-mL to 20-mL sample volumes. Slide-A-Lyzer Cassette Float Buoys are designed for floating 0.5- to 12-mL cassettes in dialysate.

Features of Slide-A-Lyzer Cassette Accessories include:
• Syringes are appropriately sized for different sample volumes
• Buoys keep stir bar from tearing dialysis membrane
• Matched to Slide-A-Lyzer Cassette for a secure fit and minimization of sample loss
• Chemically inert

Tekniske data

• Sufficient For: Floating one 0.5 mL or 3 mL Cassette with each buoy
• Content And Storage: Store at room temperature.
• Membrane: Dialysis
• Format: Cassette, Accessory
• Capacity (Metric): For 0.5 to 3 mL Cassettes
• Product Line: Slide-A-Lyzer
• Quantity: 10 Buoys
• Sample Volume (Metric): 0.5 to 3 mL
• Product Type: Cassette Float Buoy

Frequently Asked Questions (FAQs)

Does the Slide-A-Lyzer Dialysis Cassette leak?

The innovative, patented design does not permit “wicking” that can occur with homemade microdialysis devices.

What sizes are available for the Slide-A-Lyzer Dialysis Cassette? What MWCO membranes are available?

Four sizes to accommodate samples between 0.1 and 30 ml are currently available in five different MWCOs (2K, 3.5K, 7K, 10K and 20K). The G2 Slide A lyzers on the other hand are available in five different sizes to accommodate samples between 0.25 and 70mL.
These units, especially those that accommodate smaller volumes, provide for greater sample recovery and shorter dialysis times than one can achieve with traditional tubing. We have a cassette selection guide to help you choose the appropriate cassette for your application. There are also product demonstrations for this and other High-Performance Dialysis Products.

What prevents the Slide-A-Lyzer Dialysis Cassette from leaking?

An internal gasket, which isn't visible after the cassette is assembled, reseals itself as the needle is withdrawn. To maintain sample volume and integrity, users should remember these three key rules:

1. Before use, fill the cassette with air to verify that the system is airtight.
2. Puncture each porthole only once.
3. Do not allow the membrane to come into contact with the sharp needle bevel.

What is the purpose of the buoy for the Slide-A-Lyzer Dialysis Cassette?

The buoy suspends the Slide-A-Lyzer Cassette above the stirring bar during dialysis and it provides a benchtop stand on which to set the Slide-A-Lyzer Cassette. These are not required with the G2 Slide-A-Lyzer Dialysis Cassettes.

What is the highest temperature and the pH range that is compatible with the Slide-A-Lyzer Cassette?

Slide-A-Lyzer Cassettes can be held at 56 degrees C for two hours. At temperatures and times above these, the plastic gets soft and can deform. Thermo Scientific recommends that users maintain a pH range of 5-9.

Is the Slide-A-Lyzer Dialysis Cassette compatible with organic solvents?

Thermo Scientific researchers have successfully used 10% solutions of DMF, DMSO, acetonitrile, methanol, hexane and heptane; 100% acetic acid; and 70% ethanol with the Slide-A-Lyzer Cassette. The units are compatible with sample containing up to 75% acetonitrile and 1% TFA; however, Thermo Scientific recommends not dialyzing into a buffer that contains an acetonitrile concentration greater than 10%.
The casing is made of ABS plastic and the gasket is made from a non-leaching silicone-like material. The membrane is made from regenerated cellulose.

Is it necessary to extensively wash or boil the Slide-A-Lyzer Cassette before use to remove contaminants?

No. The Slide-A-Lyzer Cassette membrane requires no pretreatment. Trace metals are present in all Slide-A-Lyzer Cassette membranes. The 7,000 MWCO membranes contain 13% glycerol, 10,000 MWCO membranes contain 12% glycerol and 10,000 MWCO membranes contain 21% glycerol. These items will be lost during the dialysis process. To remove these items before introducing the sample, dialyze against water or a buffer for 10-15 minutes.

Is it necessary to buy needles and syringes from Thermo Scientific if there are already some available in the lab when using the Slide-A-Lyzer Dialysis Cassette?

No, any 18-gauge needle that is at least one-inch long and any syringe that is of sufficient volume will work.

If the Slide-A-Lyzer Dialysis Cassette is not reusable, why are there four portholes?

It is possible to add reagents to the Slide-A-Lyzer Dialysis Cassette at different times using different portholes, allow a reaction to occur, and then dialyze in the same Slide-A-Lyzer Dialysis Cassette, reducing sample loss through reduced sample handling.

How much sample will I lose in the Slide-A-Lyzer Cassette?

The Slide-A-Lyzer Cassette membrane is made of the same regenerated cellulose that is used in dialysis tubing, so a researcher should expect about the same protein loss one would experience with tubing. As a guide, a 1 mg/mL solution will generally have a recovery rate of greater than 95%. At 100µg/mL, the recovery rate drops to 75%-80%. With a dilute (e.g., 10 µg/mL) protein solution, one can lose up to 50% or more of the sample.

How long should I dialyze with the Slide-A-Lyzer Cassette?

Typically, one should dialyze for one hour at room temperature three times in dialysate that is about 300 times the sample volume. If required, dialysis may continue overnight at 4 degrees C to yield a more complete dialysis. Because each sample is different, the optimum time must be derived empirically.