NGF Recombinant Mouse Protein, Invitrogen™ Sino Biological™

This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse NGF (NP_001106168.1), residues 122 to 241, with an initial Met at the N-terminus

Product Code	Brand	Quantity
13476286	Invitrogen 50385MNAC5	5μg
13456286	Invitrogen 50385MNAC25	5 x 5μg
13486286	Invitrogen 50385MNAC50	50μg

Description

Nerve growth factor (NGF), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.

• Activity: Measured in a cell proliferation assay using TF1 human erythroleukemic cells; the ED₅₀ for this effect is 0.2-2.0ng/mL
• Formulation: Lyophilized in 50mM Na acetate buffer, pH 5.5, 150mM NaCl, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
• Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.

Specifications

• For Use With (Application): Protein Expression and Isolation, Enzyme and Protein Activity Assay, Protein Assay Control, Reference Standards and Accessories
• Molecular Weight (g/mol): 13.5kDa
• Structural Form: Native
• Gene Alias: Ngf, Ngfb
• Gene Symbol: NGF
• Expression System: Mammalian
• Form: Lyophilized
• Purity or Quality Grade: ≥95% by SDS-PAGE

• Gene ID (Entrez): 18049
• Storage Requirements: Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
• Endotoxin Concentration: <1 EU/μg
• Common Name: Nerve growth factor
• Protein Tag: Untagged
• Protein Family: Growth Factors & Receptors
• Protein Subtype: Neurotrophic Factors

For Research Use Only. Not for use in diagnostic procedures.