IL1B Recombinant Human Protein, Invitrogen™ Sino Biological™

Product Code. 15224725

Brand: Invitrogen 10139HNAE25

Description

This recombinant protein was expressed from a DNA sequence encoding the mature form of human IL1B (NP_000567.1) (Ala 117-Ser 269)

Interleukin 1, beta (IL1B), a recombinant human protein, is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications.

• Activity: 1.Measured by its binding ability in a functional ELISA; a. Immobilized recombinant human IL1B at 2μg/mL (100μL/well) can bind recombinant human IL1R2 with a linear range of 6.25-400ng/mL; b. Immobilized human IL1B at 10μg/mL (100μL/well) can bind human IL1R1, the EC₅₀ of human IL1R1 is 0.48μg/mL; 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells, the ED₅₀ for this effect is typically 0.5-5pg/mL
• Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na₂HPO₄, 1.8mM KH₂PO₄, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
• Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.

Specifications

• For Use With (Application): Protein Expression and Isolation, Enzyme and Protein Activity Assay, Protein Assay Control, Reference Standards and Accessories
• Gene ID (Entrez): 3553
• Molecular Weight (g/mol): 17kDa
• Quantity: 5 x 5 μg
• Storage Requirements: Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
• Gene Alias: IL-1, IL1-BETA, IL1F2, IL1B
• Common Name: Interleukin 1, beta
• Gene Symbol: IL1B
• Protein Tag: Untagged
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Form: Lyophilized
• Purity or Quality Grade: ≥90% by SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.