IL1A Recombinant Mouse Protein, Invitrogen™ Sino Biological™

Product Code. 15362746

Brand: Invitrogen 50114MNAE5

Description

Includes

One vial of lyophilized protein

This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse IL1A (NP_034684.2) (Ser 115-Ser 270), with an initial Met at the N-terminus

Interleukin 1 alpha (IL1A), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

• Activity: 1. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells, the ED₅₀ for this effect is typically 2-10pg/mL; 2. Measured by its binding ability in a functional ELISA; immobilized mouse IL1A at 10μg/mL (100μL/well) can bind mouse IL1R1, the EC₅₀ of mouse IL1R1 is 0.13μg/mL
• Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na₂HPO₄, 1.8mM KH₂PO₄, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
• Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.

Specifications

• For Use With (Application): Protein Expression and Isolation, Enzyme and Protein Activity Assay, Protein Assay Control, Reference Standards and Accessories
• Molecular Weight (g/mol): 17kDa
• Quantity: 5 μg
• Storage Requirements: Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
• Gene Alias: RP23-160G19.8, Il-1a
• Common Name: Interleukin 1 alpha
• Gene Symbol: IL1A
• Protein Tag: Untagged
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Form: Lyophilized
• Protein Subtype: Interleukins
• Purity or Quality Grade: ≥90% by SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.