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CD4 Recombinant Human Protein, His Tag, Invitrogen™ Sino Biological™
This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Trp 390) of human CD4 (NP_000607.1) fused to a polyhistidine tag at the C-terminus
Brand: Invitrogen 10400H08H50
Code : NEW
Additional Details : Weight : 0.01000kg
Description
Cluster of Differentiation 4 (CD4), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.- Activity: 1. Measured by its ability to bind to HIV-1 gp120 in a functional ELISA; 2. Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells; when 5 x 104 cells/well are added to CD4 coated plates (2.5μg/mL and 100μL/well), approximately 60-80% will adhere specifically after 60 minutes at 37°C
- Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
This protein is manufactured by Sino Biological Inc.
Specifications
Protein Expression and Isolation, Enzyme and Protein Activity Assay, Protein Assay Control, Reference Standards and Accessories | |
42.2kDa | |
Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed. | |
<1 EU/μg | |
CD4 molecule | |
His Tag | |
CD Proteins | |
Glycoproteins |
920 | |
50μg | |
Truncated/Recombinant/Cell Surface Protein | |
CD4mut | |
CD4 | |
Mammalian | |
Lyophilized | |
≥90% by SDS-PAGE |
For Research Use Only. Not for use in diagnostic procedures.