Sino Biological™ CD34 Recombinant Human Protein, hIgG1-Fc Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of the human CD34 precursor (NP_001020280.1) (Met 1-Thr 290) fused to the Fc region of human IgG1 at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 10103H02H50

CD34 Rec. Hu. Protein (hIgG1-Fc Tag, Act.), 50 µg

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 13452929

Quantity Price
1 £ 266.0 / 50µg
Estimated Shipment date
from Supplier 13-12-2016
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Description and Specification

Specification

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 54kDa
Purity ≥90% by SDS-PAGE
Quantity 50μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Truncated/Recombinant/Cell Surface Protein
Protein Subtype Glycoproteins
Endotoxin Concentration <1 EU/μg
Gene Alias RP11-328D5.2, CD34
Common Name CD34 molecule
Gene Symbol CD34
Protein Tag IgG1 Fc Tag
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian

Cluster of Differentiation 34 (CD34), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.
  • Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line; when 4 x 104 cells/well are added to human CD34 coated plates (100μL/well of a 0.8μg/mL solution), >40 % will adhere after one hour at 37°C
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.