Sino Biological™ CD34 Recombinant Human Protein, hIgG1-Fc Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of the human CD34 precursor (NP_001020280.1) (Met 1-Thr 290) fused to the Fc region of human IgG1 at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 10103H02H50

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 13452929

Quantity Price
1 £ 266.0 / 50µg
Estimated Shipment date
from Supplier 15-05-2017
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Description and Specification

Specification

Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Purity ≥90% by SDS-PAGE
Molecular Weight 54kDa
Protein Tag IgG1 Fc Tag
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Endotoxin Concentration <1 EU/μg
Gene Alias RP11-328D5.2, CD34
Expression System Mammalian
Validated Application Assay Standard
Common Name CD34 molecule
Protein Subtype Glycoproteins
Quantity 50μg
Gene Symbol CD34
Format Lyophilized
Protein Family CD Proteins
Structural Form Truncated/Recombinant/Cell Surface Protein

Cluster of Differentiation 34 (CD34), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.
  • Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line; when 4 x 104 cells/well are added to human CD34 coated plates (100μL/well of a 0.8μg/mL solution), >40 % will adhere after one hour at 37°C
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.