SingleStep Poly-HRP anti-Rabbit IgG (H+L) Secondary Antibody

Goat Polyclonal Antibody

Product Code	Brand	Quantity
15951448	EnquireBio Q2AB2-100UG	100 μg
15981448	EnquireBio Q2AB2-500UG	500 μg
15971448	EnquireBio Q2AB2-1MG	1 mg
15901458	EnquireBio Q2AB2-5MG	5 mg
15911458	EnquireBio Q2AB2-5ML	5 mL
15961448	EnquireBio Q2AB2-10ML	10 mL
15991448	EnquireBio Q2AB2-50ML	50 mL
15941448	EnquireBio Q2AB2-100ML	100 mL

Description

SingleStep™ Poly-HRP (Horse Radish Peroxidase) conjugated goat anti-rabbit secondary antibodies provide signal amplification over traditional HRP conjugated reagents in immunohistochemistry (IHC) staining using primary antibodies from a rabbit host. The signal amplification afforded by these HRP polymer conjugated antibodies is sufficient to enable application of a single secondary antibody unlike standard HRP conjugated reagents which utilize an unconjugated or biotinylated secondary antibody and then an additional HRP conjugated secondary antibody or HRP conjugted streptavidin to visualize staining. These additional staining steps increase background staining and variability between samples which is obviously undesirable in IHC. SingleStep™ goat anti-rabbit poly-HRP secondary antibodies are sourced from the industry leading secondary antibody manufacturer and are then conjugated to a carefully controlled number of HRP molecules to facilitate excellent tissue penetration without sacrificing signal amplification. Our predilute products also incorporate our enhanced IHC staining buffer which contains optimized stabilizers and blocking reagents to ensure maximum sensitivity and high reproducibility in IHC in most sample types.

Specifications

• Antigen: IgG (H+L)
• Classification: Polyclonal
• Host Species: Goat
• Target Species: Rabbit
• Immunogen: Generation of this anti-Poly-HRP Anti-Rabbit IgG (H+L) Secondary Antibody antibody was achieved by immunizing a Goat host with Pooled population of Rabbit IgG
• Purification Method: Antibody is initially purified by ammonium sulfate precipitation followed by cross-adsorption to ensure minimal cross-reactivity to human, bovine, and equine antibodies. Purification of SingleStep(TM) poly-HRP conjugated goat anti-rabbit secondary is achieved via size exclusion liquid chromatography.

• Applications: Immunohistochemistry
• Conjugate: HRP
• Regulatory Status: RUO
• Formulation: Pre-dilute antibody is in proprietary signal enhancement buffer composed of protein based blocking agent (non-BSA), non-ionic detergent, preservative (non-azide), and HRP-stabilizer at pH 7.4. Concentrated format also contains 50% glycerol.
• Primary or Secondary: Secondary
• Content And Storage: Store at 4°C. Do not freeze. Stable for >1 year if stored properly.